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The \"Technology Workshop on Flow Cytometry,\" held from October 30^th to November 1^st , 2023, at the Dr. D. Y. Patil Medical College, Hospital and Research Centre in Pune, India, was a comprehensive and enlightening event focused on the principles and practical applications of flow cytometry. The workshop was organized by Dr Srikanth Tripathy, Director of Medical Research, and coordinated by Dr Poonam Suryawanshi, Dr Priyanka Khopkar-Kale and Bhagyashri Takbhate of the Central Research Facility. A detail programme of the workshop as under: -

\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n

\n Day 1 – 30^th October 2023 \n \n
\n Time \n	\n Session \n	\n Speaker/Demonstrator \n	\n Venue \n
\n 02:00pm -02.30pm \n	\n Registration and Welcome address \n	\n Dr Srikanth Tripathy, \n Bhagyashri Takbhate \n & Nitin Deshpande \n CRF, DYPMCHRC \n	\n Lecture Hall -2, 2^nd floor, Dr D Y Patil Medical College, Hospital and Research Centre \n
\n 02.30pm - 03.15pm \n	\n Lecture-Introduction to flow cytometry-Fluidics, optics, and lasers, Detectors and signal processing. \n Applications of flow cytometer \n	\n Dr Anjan Ghosh, Thermo Fisher Scientific Pvt Ltd \n
\n 03.15pm - 04.00pm \n	\n Lecture- Introducing Attune Cytpix-The Next level in Flowcytometry. \n	\n Dr Saket Siddharth, Thermo Fisher Scientific Pvt Ltd \n
\n 04.00pm - 04.15pm \n	\n Tea Break \n
\n 04.15pm - 05.00pm \n	\n Lab Demonstration- Demonstration of Attune Cytpix - Features highlight: Ease of Instrument Setup \n	\n Dr Saket Siddharth, Thermo Fisher Scientific Pvt Ltd \n	\n Central Research Facility, 5^th Floor, Dr D Y Patil Medical College, Hospital and Research Centre \n

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\n Time \n	\n Session \n	\n Speaker/Demonstrator \n	\n Venue \n
\n \n Day 2 – 31^st October 2023 \n
\n 01.00pm - 02.00pm \n	\n Practical Demonstration - Instrument set-up for Attune Cytpix, calibration, and compensation settings in multicolour assays- Significance of compensation and how to perform it \n	\n Dr Saket Siddharth, Thermo Fisher Scientific Pvt Ltd \n Dr Poonam Suryawanshi, \n Research Scientist, CRF, DYPMCHRC \n Bhagyashri Takbhate \n Research Fellow, CRF, DYPMCHRC \n	\n Central Research Facility, 5^th Floor, Dr D Y Patil Medical College, Hospital and Research Centre \n
\n 2.00pm-3.00pm \n	\n Practical Demonstration-Immunophenotyping of the cells including T cells, B cells, NK cells and memory subsets of T cells \n
\n 3.00pm-3.15pm \n	\n Tea Break \n
\n 3.15pm-4.00pm \n	\n Practical Demonstration - Lineage classification and immunophenotyping of human haematolymphoid malignant neoplasms \n
\n 4.00pm-4.45pm \n	\n Practical Demonstration – Assessment of Mesenchymal stem cell differentiation using surface markers. \n
\n 4.45pm-5.00pm \n	\n Questions and Answers \n

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\n Time \n	\n Session \n	\n Speaker/Demonstrator \n	\n Venue \n
\n Day 3 – 01^st November 2023 \n
\n 1.00pm – 2.00pm \n	\n Practical Demonstration - Apoptosis analysis using Annexin V &Propidium Iodide through flow cytometry \n	\n Dr Saket Siddharth, Thermo Fisher Scientific Pvt Ltd \n Dr Poonam Suryawanshi, \n Research Scientist, CRF, DYPMCHRC \n Bhagyashri Takbhate \n Research Fellow, CRF, DYPMCHRC \n	\n Central Research Facility, 5^th Floor, Dr D Y Patil Medical College, Hospital and Research Centre \n
\n 2.00pm-3.00pm \n	\n Practical Demonstration- Assessment of oral cancer cell line using surface markers \n
\n 3.00pm-3.15pm \n	\n Tea Break \n
\n 3.15pm-4.30pm \n	\n Practical Demonstration –Analysis of Flow Data and Reporting Results—Discussion about Fundamental Statistics \n
\n 4.30pm-5.00pm \n	\n Discussion, Questions and Answers, Closing Remark and Feedback \n

The primary purpose of the workshop was to provide participants with a comprehensive understanding of flow cytometry, its fundamental principles, practical applications, and advanced technologies. The workshop aimed to equip participants with the knowledge and skills necessary to effectively use flow cytometry in various fields, including immunology, cancer research, microbiology, and stem cell biology.

The three-day workshop featured a diverse range of activities, including lectures, practical demonstrations, and interactive sessions, all designed to provide participants with a deep understanding of flow cytometry technology and its versatile applications.

Dr Srikanth Tripathy emphasized the importance of flow cytometry in cellular analysis and its role in scientific discovery. Dr Anjan Ghosh from Thermo Fisher Scientific provided an introduction to flow cytometry, covering fluidics, optics, lasers, detectors, and signal processing, along with its applications.

Dr Saket Siddharth introduced the Attune Cytpix Flow Cytometer, followed by a practical demonstration of its operation, including lasers, detectors, optics, and performance testing. Practical demonstrations covered immunophenotyping of PBMCs, mesenchymal stem cell differentiation assessment, cell apoptosis analysis using Annexin V/ Propidium Iodide kit and lineage classification of haematolymphoid malignancies using a variety of sample types and specific antibodies. Dr Saket Siddharth also discussed data analysis and presentation for flow cytometry data, providing essential knowledge for scientific communication.

The workshop introduced participants to the capabilities of the Invitrogen Attune CytPix Flow Cytometer, combining acoustic focusing with high-speed brightfield imaging. This technology allowed for enhanced understanding of cell morphology, gating accuracy confirmation, capturing cell-to-cell interactions, and visualizing structural features of large cell populations.

In summary, the \"Technology Workshop on Flow Cytometry\" proved to be an invaluable educational experience, equipping participants with the knowledge and practical skills needed to harness the power of flow cytometry in their scientific endeavours. The event fostered collaboration and knowledge sharing, ultimately advancing the world of scientific discovery in fields ranging from immunology to cancer research, microbiology to stem cell biology.

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Y. Patil Medical College, Hospital and Research Centre, Pimpri, Pune, provided participants with in-depth knowledge and hands-on experience in flow cytometry. From fundamental principles to advanced applications and data analysis, the workshop explored the power of this technology in various scientific fields. The workshop was organized by Dr. Srikanth Tripathy, Director of Medical Research, and coordinated by Dr. Poonam Suryawanshi, Dr. Priyanka Khopkar-Kale, and Bhagyashri Takbhate of the Central Research Facility.\n\n## Highlights\n\n* **Expert Speakers:** Learned from leading experts, including Dr. Srikanth Tripathy (Director of Medical Research), Dr. Anjan Ghosh (Thermo Fisher Scientific), and Dr. Saket Siddharth (Thermo Fisher Scientific).\n* **Comprehensive Curriculum:** Covered fluidics, optics, lasers, detectors, signal processing, data analysis, and diverse applications of flow cytometry. Special emphasis was given to the Attune CytPix Flow Cytometer, combining acoustic focusing with high-speed brightfield imaging for enhanced cellular analysis.\n* **Hands-on Demonstrations:** Gained practical experience with the Attune CytPix Flow Cytometer, including instrument setup, calibration, compensation, and various assays such as immunophenotyping of PBMCs, mesenchymal stem cell differentiation assessment, cell apoptosis analysis using Annexin V/ Propidium Iodide kit and lineage classification of haematolymphoid malignancies.\n* **Real-world Applications:** Explored immunophenotyping, stem cell differentiation, apoptosis analysis, and lineage classification of hematolymphoid malignancies, and assessment of oral cancer cell lines using surface markers.\n* **Interactive Sessions:** Engaged in discussions and Q&A sessions with experts, fostering collaborative learning.\n\n## Detailed Schedule\n\n**Day 1 (October 30th, 2023):**\n* Registration and Welcome Address\n* Introduction to flow cytometry principles, fluidics, optics, lasers, detectors, signal processing, and applications.\n* Introduction and Demonstration of the Attune CytPix Flow Cytometer – features and setup.\n\n**Day 2 (October 31st, 2023):**\n* Practical demonstration: Attune CytPix instrument setup, calibration, and compensation settings in multicolour assays- Significance of compensation and how to perform it.\n* Practical demonstration: Immunophenotyping of cells including T cells, B cells, NK cells and memory subsets of T cells and lineage classification of human haematolymphoid malignant neoplasms.\n* Practical demonstration: Assessment of mesenchymal stem cell differentiation using surface markers.\n\n**Day 3 (November 1st, 2023):**\n* Practical demonstration: Apoptosis analysis using Annexin V & Propidium Iodide.\n* Practical demonstration: Assessment of oral cancer cell lines using surface markers.\n* Practical demonstration: Flow data analysis, reporting results, and discussion about Fundamental Statistics.\n* Closing remarks and feedback session.\n\n## Faculty\n\n* **Dr. Srikanth Tripathy:** Director of Medical Research, DYPMCHRC\n* **Dr. Poonam Suryawanshi:** Research Scientist, Central Research Facility (CRF), DYPMCHRC\n* **Dr. Priyanka Khopkar-Kale:** CRF, DYPMCHRC\n* **Bhagyashri Takbhate:** Research Fellow, CRF, DYPMCHRC\n* **Dr. Anjan Ghosh:** Thermo Fisher Scientific Pvt Ltd\n* **Dr. Saket Siddharth:** Thermo Fisher Scientific Pvt Ltd\n* **Nitin Deshpande:** CRF, DYPMCHRC\n\n## Who Should Attend?\n\nResearchers, clinicians, and students interested in learning about flow cytometry and its applications. This workshop is highly relevant for those working in immunology, oncology, microbiology, and stem cell biology. \n\n## Learning Objectives\n\nUpon completion of this workshop, participants will be able to:\n\n* Understand the principles and applications of flow cytometry.\n* Operate the Attune CytPix Flow Cytometer.\n* Design and perform flow cytometry experiments, including sample preparation and data acquisition.\n* Analyze and interpret flow cytometry data using appropriate software and statistical methods.\n* Apply flow cytometry techniques to their respective research or clinical areas.\n* Troubleshoot common issues encountered in flow cytometry experiments.\n\n## Venue\n\nDr. D. Y. Patil Medical College, Hospital and Research Centre, Pimpri, Pune\n\n## Organizers\n\nCentral Research Facility, DYPMCHRC","sdg",[107,108,109,110],"2025-02-23T14:32:08.112835+05:30","2025-02-23T23:13:08.093644+05:30",{"_59":155,"_112":156,"_114":157,"_116":158,"_23":157,"_119":159,"_121":160},{"_59":141,"_112":142,"_114":143,"_116":144,"_23":143,"_119":145,"_121":146},{"_59":130,"_112":131,"_114":132,"_116":133,"_23":134,"_119":135,"_121":136},{"_59":111,"_112":113,"_114":115,"_116":117,"_23":118,"_119":120,"_121":122},"https://cdn.openscholar.in/media/public/sdg/assets/E-WEB-Goal-17.png","targets",[123],"sdg_name","Partnerships for the Goals","sdg_number",17,"Partnerships For The Goals","long_description","Strengthen the means of implementation and revitalize the Global Partnership for Sustainable Development.","sdg_justification","The workshop exemplifies partnerships between academia and industry, which are crucial for driving progress towards the SDGs. 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all.",{"_211":260,"_209":309,"_223":90,"_207":220,"_215":220,"_213":220,"_219":220,"_221":220,"_217":220,"_222":220},"https://cdn.openscholar.in/media/public/sdg/assets/E-WEB-Goal-05.png","https://cdn.openscholar.in/media/public/sdg/assets/background/Rectangle_6002.png","Gender Equality","#ff3a21","Achieve gender equality and empower all women and girls.",{"_211":329,"_209":330,"_207":331,"_215":253,"_217":262,"_213":218,"_222":235,"_223":235,"_219":220,"_221":220},229,74,71,"https://cdn.openscholar.in/media/public/sdg/assets/background/Rectangle_6000.png","#c5192d",{"_207":335,"_209":336,"_211":337,"_213":338,"_215":339,"_217":340,"_223":272,"_221":235,"_222":90,"_219":220},888,522,440,104,58,31,"https://cdn.openscholar.in/media/public/sdg/assets/background/Rectangle_5999.png","#4c9f38",{"_211":344,"_207":345,"_209":346,"_217":347,"_213":348,"_222":349,"_215":233,"_219":252,"_221":262,"_223":90},6282,1598,564,175,161,27,"https://cdn.openscholar.in/media/public/sdg/assets/E-WEB-Goal-02.png","https://cdn.openscholar.in/media/public/sdg/assets/background/image_1053_1.png","Zero Hunger","#dda83a","End hunger, achieve food security and improved nutrition and promote sustainable agriculture.",{"_211":306,"_209":282,"_207":349,"_217":133,"_213":218,"_223":90,"_215":220,"_219":220,"_221":220,"_222":220},"https://cdn.openscholar.in/media/public/sdg/assets/E-WEB-Goal-01_z15joxf.png","https://cdn.openscholar.in/media/public/sdg/assets/background/image_1051.png","No Poverty","#e5243b","End poverty in all its forms everywhere.",{"_211":362,"_207":158,"_223":235,"_215":90,"_217":90,"_213":220,"_219":220,"_221":220,"_222":220,"_209":220},68]